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Updated: Sep 21, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
NT-seq: a chemical-based sequencing method for genomic methylome profiling
Xuwen Li1, Shiyuan Guo2, Yan Cui1
1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Abstract:
DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N6-methyladenine (6mA), N4-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation.
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