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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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DIPA-CRISPR is a simple and accessible method for insect gene editing.

Yu Shirai1, Maria-Dolors Piulachs2, Xavier Belles2

  • 1Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.

Cell Reports Methods
|May 31, 2022
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Summary

Direct parental CRISPR (DIPA-CRISPR) allows efficient insect gene editing by injecting Cas9 ribonucleoproteins into adult females. This accessible method overcomes limitations of embryonic microinjection, enabling applications in diverse insect species.

Keywords:
CRISPR-Cas9DIPA-CRISPRbeetlescockroachesgene editinggenome editing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Entomology

Background:

  • Conventional insect gene editing relies on microinjection into early embryos, limiting its application to species with accessible reproductive systems.
  • This technique is not feasible for many insect species, hindering broader genetic research and application.

Purpose of the Study:

  • To develop a simple, accessible, and broadly applicable method for insect gene editing.
  • To overcome the limitations of traditional embryonic microinjection techniques.

Main Methods:

  • Developed "direct parental" CRISPR (DIPA-CRISPR), a novel gene editing approach.
  • Injected Cas9 ribonucleoproteins (RNPs) into the haemocoel of adult female insects.
  • Utilized commercially available standard Cas9 protein for practicality.

Main Results:

  • DIPA-CRISPR efficiently introduced heritable mutations in developing oocytes.
  • Demonstrated high efficiency in gene editing in cockroaches, a species previously challenging for conventional methods.
  • Successfully applied DIPA-CRISPR in the model beetle *Tribolium castaneum*.

Conclusions:

  • DIPA-CRISPR is a practical and feasible method for insect gene editing.
  • This technique significantly expands the scope of gene editing applications across a wide range of insect species.
  • The accessibility of DIPA-CRISPR facilitates broader research in insect genetics and biotechnology.