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Published on: July 6, 2016
A dicyanoisophorone-based fluorescent probe for hypochlorite with a fast response and its applications in bioimaging
Chenming Chan1, Jia Li1, Zhaoli Xue1
1School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. zhaolixue@ujs.edu.cn.
Abstract:
One kind of phenolic substituted dicyanoisophorone derivative (Is-OL) has been designed and successfully synthesized for the detection of hypochlorite in water samples, test strips and living HeLa cells. The probe Is-OL showed high sensitivity and selectivity to hypochlorite over other competitive ROS and metal ions. Moreover, Is-OL can react instantaneously with hypochlorite (<5 s) while exhibiting a significant color change from yellow to colorless, which makes "naked-eye" detection possible with a low detection limit (0.095 μM). The results based on water tests and living HeLa cell experiments showed that Is-OL could be applied as a potential candidate for the detection of hypochlorite.
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