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Updated: Jun 18, 2026

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Core-Shell Microfibers via Bioorthogonal Layer-by-Layer Assembly.

Anitha Ravikrishnan¹, He Zhang¹, Joseph M Fox²

¹Department of Materials Science and Engineering, University of Delaware, Newark, Delaware 19716, United States.

ACS Macro Letters

|May 31, 2022

Summary

This summary is machine-generated.

Researchers developed core-shell microfibers using bioorthogonal chemistry for biomedical uses. These scaffolds support fibroblast growth and may treat vocal fold scarring.

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Area of Science:

Biomaterials Science
Tissue Engineering
Bioorganic Chemistry

Background:

Developing advanced scaffolds is crucial for tissue regeneration.
Core-shell microfiber structures offer unique properties for biomedical applications.
Bioorthogonal chemistry provides precise methods for material functionalization.

Purpose of the Study:

To create novel core-shell microfibers using electrospinning and bioorthogonal chemistry.
To functionalize these scaffolds with specific biomolecules for enhanced cellular interaction.
To evaluate the potential of these scaffolds for vocal fold tissue engineering.

Main Methods:

Fabrication of poly(ε-caprolactone) (PCL) core microfibers via electrospinning.
Surface modification with tetrazine groups for subsequent bioorthogonal reactions.
Layer-by-layer deposition of hyaluronic acid (HA) using tetrazine (Tz) and trans-cyclooctene (TCO) click chemistry.
Covalent attachment of RGD peptides to impart integrin-binding motifs.

Main Results:

Successfully constructed core-shell microfibers with a PCL core and a cross-linked HA shell.
Demonstrated controlled growth of the HA gel layer around individual microfibers.
Showed that scaffolds support primary porcine vocal fold fibroblast attachment and growth.
Observed no significant induction of the myofibroblast phenotype, with moderate effects of TGF-β stimulation and Y27632 inhibition.

Conclusions:

The developed bioorthogonally assembled core-shell microfibers show promise for biomedical applications.
These scaffolds provide a suitable microenvironment for vocal fold fibroblasts.
The PCL core and HA shell structure may be beneficial for therapeutic implants targeting vocal fold scarring.