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Association of Nucleostemin Polymorphisms with Chronic Hepatitis B Virus Infection in Chinese Han Population

Jixia Li1, Jinya Xu2, Yangui Wang1

  • 1Department of Clinical Laboratory, Yantaishan Hospital, Yantai, China.

Genetic Testing and Molecular Biomarkers
|May 31, 2022
PubMed

Insights

Genetic variants in the nucleostemin (NS) gene are linked to chronic hepatitis B virus infection (CHB) risk in the Chinese Han population. These NS gene variations may serve as potential biomarkers for CHB susceptibility.

Area of Science:

  • Genetics
  • Hepatology
  • Infectious Diseases

Background:

  • Chronic hepatitis B virus infection (CHB) presents a significant global health and economic challenge.
  • Host genetic factors are recognized as crucial determinants of CHB susceptibility and disease progression.
  • The role of specific gene variants in CHB pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the association between genetic variations in the nucleostemin (NS) gene and the susceptibility to CHB.
  • To identify potential genetic biomarkers for CHB risk within the Chinese Han population.

Main Methods:

  • Genotyping of four single nucleotide polymorphisms (SNPs) in the NS gene among 446 CHB patients and 399 healthy controls.
  • Utilized polymerase chain reaction-ligation detection reaction (PCR-LDR) for SNP analysis.
  • Performed Bonferroni correction and gender-specific analyses for statistical validation.

Main Results:

  • Three SNPs (rs3733039, rs1866268, rs11177) in the NS gene showed significant association with CHB.
  • The association of rs3733039 with CHB remained significant after Bonferroni correction.
  • Gender-specific analysis revealed associations in both male and female subgroups, with distinct SNP associations observed between genders after multiple comparison corrections.
  • Haplotype analysis identified significant associations for C-C-G and T-T-T haplotypes within the rs3733039-rs1866268-rs11177 block.

Conclusions:

  • This study provides the first evidence of a genetic association between NS gene SNPs and CHB risk in the Chinese Han population.
  • Variations within the NS gene may represent novel genetic biomarkers for predicting CHB susceptibility.
  • Further research is warranted to explore the functional implications of these NS gene variants in CHB pathogenesis.

Keywords:
associationchronic hepatitis B virus infectionnucleosteminsingle nucleotide polymorphisms

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