Chryseobacterium faecale sp. nov., isolated from camel feces

Strain F4^T (=KACC 22401^T=JCM 34836^T), a novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, was isolated from camel (Camelus bactrianus) faeces. The newly identified bacterial strain F4^T was grown in Reasoner's 2A medium [0-2 % (w/v) NaCl (optimum, 0 %), pH 7.0-8.0 (optimum, pH 7.0), and 18-40 °C (optimum, 30 °C)]. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain F4^T belonged to the genus Chryseobacterium, with its closest neighbours being Chryseobacterium haifense DSM 19056^T (98.0 %), Chryseobacterium anthropi CCUG 52764^T (97.3 %), Chryseobacterium montana WG4^T (95.7 %) and Chryseobacterium koreensis Chj70^T (94.7 %). Complete genome sequence of strain F4^T was obtained using a hybrid assembly pipeline integrating sequences obtained using both the Oxford Nanopore and Illumina platforms. Genomic comparisons of strain F4^T with type species in the genus Chryseobacterium were conducted using digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity, resulting in values of ≤20.5, ≤77.9 and ≤80.8 %, respectively. The genomic DNA G+C content of type strain F4^T was 39.7 mol%. The major fatty acids of the strain F4^T were anteiso-C_15 : 0 and iso-C_18 : 3, and MK-6 was its major respiratory quinone. Moreover, the major polar lipid of strain F4^T was phosphatidylethanolamine. The genome of strain F4^T harbours only one antibiotic resistance gene (blaCME-1) encoding a β-lactamase, which attributes β-lactam antibiotic resistance. Based on the results of our chemotaxonomic, genotypic and phenotype analyses, strain F4^T is identified as a novel species of the genus Chryseobacterium, for which the name Chryseobacterium faecale sp. nov. is proposed.