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Updated: Sep 21, 2025

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
Published on: November 4, 2021
Evaporation driven smart patterning of microparticles on a rigid-soft composite substrate.
Rameez Iqbal1, Atsushi Matsumoto2, Daniel Carlson2
1Micro/Bio/Nanofluidics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1, Tancha, Onna-san, Okinawa 904-0495, Japan; Micro-Nano-Biofluidics Unit, Indian Institute of Technology Madras, Chennai 600036, India.
Researchers developed a new method for microparticle patterning using 3D printing and soft lithography on composite polydimethylsiloxane (PDMS) substrates. This technique allows for controlled deposition of microparticles in specific patterns by tuning the ratio of rigid and soft regions on the substrate.
Area of Science:
- Materials Science
- Microfluidics
- Surface Science
Background:
- Liquid droplet behavior on surfaces is influenced by substrate properties, such as rigidity.
- Controlling droplet receding contact angles is crucial for applications like microparticle deposition.
- Existing methods for microparticle patterning often rely on complex lithographic techniques.
Purpose of the Study:
- To investigate the effect of composite rigid-soft polydimethylsiloxane (PDMS) substrates on microparticle deposition during droplet evaporation.
- To develop a novel, simpler method for creating controlled microparticle patterns using 3D printing and soft lithography.
- To demonstrate the tunability of microparticle deposition patterns by varying the substrate's rigid-soft ratio.
Main Methods:
- Fabrication of composite PDMS substrates with varying rigid-soft ratios and core shapes using 3D printing and soft lithography.
- Deposition of a sessile suspension droplet containing spherical microparticles onto the composite substrate.
- Analysis of droplet evaporation dynamics and resulting microparticle deposition patterns under ambient conditions.
Main Results:
- Achieved evaporation-induced microparticle patterning in circular, triangular, and rectangular areas ranging from microns to millimeters.
- Demonstrated the ability to control deposition size and shape from an identical droplet by altering the rigid-soft region ratio.
- Successfully created diverse microparticle patterns without relying on traditional lithographically patterned substrates.
Conclusions:
- The developed methodology offers a versatile and accessible approach for microparticle patterning.
- Composite rigid-soft PDMS substrates provide a tunable platform for controlling microparticle assembly.
- This technique opens new possibilities for fabricating microstructures and functional materials.

