Conjugated Molecule-Assisted Supramolecular Hydrogel with Enhanced Antibacterial and Antibiofouling Properties

The hydrogel using natural and synthetic polymers to create a cross-linking network has drawn attention in diverse bioapplications. However, inhibition of bacterial infection is still a challenge for hydrogel's wide application. In this work, we reported a supramolecular hydrogel with a good antibacterial property built from conjugated molecules. The water-soluble molecular 4,7-bis[9,9-di(2-carboxy-ethyl)-fluoren-2-yl]-2,1,3-benzothiadiazole (OFBTCOOH) physically linked with monomers via hydrophobic interaction. The free-radical polymerized poly(N-acryloyl glycinamide) was hydrogen-bond cross-linked by dual amides in the side chains to form a hydrogel. An adjustable micro-network was obtained by increasing OFBTCOOH with evidence of enhanced intermolecular interaction. The successfully integrated OFBTCOOH could be excited upon light irradiation. The energy of triplet-state excitons of OFBTCOOH transferred to the ground-state oxygen to produce singlet oxygen, which endowed the hydrogel with the antibacterial property. Meanwhile, the superhydrophilic surface of the hydrogel can bind water molecules to form a stable hydration layer, which acted as barriers to resist protein and bacterial adsorption and achieve the anti-biofouling goal. The ease in introducing conjugated polyelectrolytes may provide a formulation to functionalize hydrogels via various physical interactions.