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Updated: Sep 21, 2025

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
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Natural antisense RNA Foxk1-AS promotes myogenic differentiation by inhibiting Foxk1 activity

Chun Li1,2, Hao Shen3, Meng Liu3

  • 1Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 201204, People's Republic of China.

Cell Communication and Signaling : CCS
|June 1, 2022
PubMed
Abstract

Insights

Natural antisense RNA (Foxk1-AS) regulates muscle stem cell function. Foxk1-AS promotes myoblast differentiation and muscle regeneration by inhibiting Foxk1 expression.

Area of Science:

  • Molecular Biology
  • Muscle Stem Cell Biology
  • RNA Biology

Background:

  • Natural antisense RNAs regulate gene expression but their roles in muscle stem cells are unclear.
  • Muscle stem cell maintenance and myogenesis are critical for muscle repair.

Purpose of the Study:

  • To investigate the role of natural antisense RNAs in muscle stem cell myogenesis.
  • To identify novel antisense RNAs involved in muscle regeneration.

Main Methods:

  • Overexpression and knockdown of Foxk1-AS in C2C12 myoblasts and mouse muscle tissue.
  • Induction of muscle injury using BaCl2.
  • Assessment of myogenesis and regeneration using molecular and histological techniques.

Main Results:

  • A novel natural antisense RNA, Foxk1-AS, was identified, regulating Foxk1 expression.
  • Foxk1-AS overexpression inhibited Foxk1, promoting myoblast differentiation and muscle regeneration.
  • Foxk1-AS promoted Mef2c expression, a key transcription factor in myogenesis.

Conclusions:

  • Foxk1-AS acts as a repressor of Foxk1, enhancing Mef2c activity.
  • Foxk1-AS promotes myogenic differentiation and muscle fiber regeneration.
  • This study reveals a novel regulatory mechanism in muscle stem cell biology.
Keywords:
Foxk1Foxk1-ASMef2cMyogenic differentiationNatural antisense RNA

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