Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.5K
CRISPR01:59

CRISPR

53.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.1K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

443
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
443
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

152
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
152
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.2K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted mutagenesis and base editing using engineered <i>Brevibacillus laterosporus</i> Cas9 with expanded target scope in rice.

Plant biotechnology (Tokyo, Japan)·2026
Same author

Immune aging biomarkers for clinical trials.

Nature medicine·2026
Same author

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same author

Laparoscopic Splenectomy for Sclerosing Angiomatoid Nodular Transformation of the Spleen after a Whipple Procedure: A Case Report.

Surgical case reports·2026
Same author

Orbital-Engineered Sn/RuO<sub>2</sub> Nanocatalyst with Self-Regulating Electron Configuration for Durable Chlorine Evolution at Industrial Current Densities.

ACS applied materials & interfaces·2026
Same author

Structure-guided development of a potent human B<sup>0</sup>AT1 inhibitor effective in a mouse model of phenylketonuria.

Communications biology·2026

Related Experiment Video

Updated: Sep 21, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.9K

Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex.

Kazuki Kato1, Wenyuan Zhou2, Sae Okazaki1

  • 1Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

Cell
|June 1, 2022
PubMed
Summary

The Cas7-11 CRISPR-Cas system offers dual RNA targeting capabilities for bacterial and mammalian cells. Structural insights enabled engineering a compact Cas7-11 variant for in vivo RNA knockdown applications using AAV vectors.

Keywords:
CRISPRCas7-11RNA targetingcryo-EM

More Related Videos

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.6K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.1K

Related Experiment Videos

Last Updated: Sep 21, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.9K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.6K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.1K

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Gene Editing Technologies

Background:

  • The Type III-E CRISPR-Cas effector, Cas7-11, possesses unique dual RNase activities.
  • These activities include precursor CRISPR RNA (pre-crRNA) processing and CRISPR RNA (crRNA)-guided target RNA cleavage.
  • Cas7-11 represents a novel platform for RNA targeting in both bacterial and mammalian systems.

Purpose of the Study:

  • To determine the high-resolution structure of the Cas7-11 complex bound to crRNA and target RNA.
  • To elucidate the molecular mechanisms underlying Cas7-11's dual RNase activities.
  • To engineer a compact Cas7-11 variant for in vivo applications.

Main Methods:

  • Cryoelectron microscopy (cryo-EM) at 2.5-Å resolution.
  • Biochemical assays to confirm catalytic activity.
  • Rational protein engineering of Cas7-11.

Main Results:

  • The study revealed the modular architecture of Cas7-11, comprising seven domains and four linkers.
  • Structural analysis identified the roles of specific domains in crRNA processing and target RNA recognition.
  • Biochemical data correlated with structural findings regarding catalytic residue positioning for RNA cleavage.
  • A compact Cas7-11 variant (Cas7-11S) was successfully engineered.

Conclusions:

  • The determined structure provides critical insights into Cas7-11's mechanism of action for RNA targeting.
  • Engineered Cas7-11S facilitates single-vector AAV packaging for efficient transcript knockdown in human cells.
  • This work enables future in vivo applications of the Cas7-11 system for RNA modulation.