Interaction between MdWRKY55 and MdNAC17-L enhances salt tolerance in apple by activating MdNHX1 expression

Salt stress greatly hinders plant growth and development, as well as crop production. To expand the planting area and choose salt-resistant varieties of apple (Malus×domestica), it is necessary to elucidate the salt-resistance mechanisms. Here, we identified a salt-responsive WRKY transcription factor, MdWRKY55. The overexpression of MdWRKY55 in apple calli significantly improved salt tolerance. MdWRKY55 bound to the MdNHX1 promoter, thereby enhancing its transcription. MdNAC17-L significantly promoted the effect of MdWRKY55 on the expression of downstream MdNHX1 by forming a protein complex. The functional analysis of MdWRKY55 provided valuable insights into the apple salt-tolerance regulatory network and established a theoretical basis for the molecular breeding of salt-tolerant apple.