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Related Concept Videos

The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Replication in Eukaryotes02:31

Replication in Eukaryotes

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Overview
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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Replication in Prokaryotes01:32

Replication in Prokaryotes

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
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The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1.

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CMG helicase disassembly is essential and driven by two pathways in budding yeast.

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A common mechanism for recruiting the Rrm3 and RTEL1 accessory helicases to the eukaryotic replisome.

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An acidic loop in the forkhead-associated domain of the yeast meiosis-specific kinase Mek1 interacts with a specific motif in a subset of Mek1 substrates.

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Related Experiment Video

Updated: Sep 21, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

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Replicon-seq: seeing is believing.

Cristian Polo Rivera1, Tom D Deegan2

  • 1Medical Research Council (MRC) Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.

Trends in Genetics : TIG
|June 1, 2022
PubMed
Summary
This summary is machine-generated.

Claussin et al. developed Replicon-seq, a novel genome-wide DNA sequencing method. This technology enables high-resolution, in vivo monitoring of individual DNA replisome progression during replication.

Keywords:
CMG helicaseDNA replicationNanopore sequencingReplicon-seqreplisome

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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Understanding DNA replication is crucial for cell biology.
  • Existing methods have limitations in tracking individual replication events in vivo.
  • High-resolution monitoring of DNA replisomes is needed.

Purpose of the Study:

  • To introduce Replicon-seq, a new technology for genome-wide DNA sequencing.
  • To enable high-resolution, in vivo monitoring of individual replisome progression.

Main Methods:

  • Development of Replicon-seq, a novel genome-wide DNA sequencing technology.
  • Application of Replicon-seq for in vivo analysis of DNA replication dynamics.

Main Results:

  • Replicon-seq provides high-resolution data on individual replisome progression.
  • The technology allows for genome-wide monitoring of DNA replication in vivo.

Conclusions:

  • Replicon-seq is a powerful new tool for studying DNA replication.
  • This technology offers unprecedented insights into the dynamics of individual replisomes in vivo.