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Related Concept Videos

Proteomics01:33

Proteomics

8.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.0K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.9K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Related Experiment Video

Updated: Sep 21, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

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MS-based technologies for untargeted single-cell proteomics.

Lir Beck1, Tamar Geiger2

  • 1Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel.

Current Opinion in Biotechnology
|June 1, 2022
PubMed
Summary
This summary is machine-generated.

Mass spectrometry-based single-cell proteomics offers a new way to study cellular functions, overcoming limitations of genomics and transcriptomics. This technology promises to advance biomedical research and understanding of biological systems.

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Area of Science:

  • Proteomics
  • Cellular Biology
  • Biomedical Research

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity.
  • Genomics and transcriptomics provide limited insight into cellular functions.
  • Traditional single-cell protein analysis relied on antibody-based methods.

Purpose of the Study:

  • To review recent advancements in mass spectrometry-based single-cell proteomics.
  • To discuss the challenges and opportunities in this emerging field.
  • To highlight the potential impact on biomedical research.

Main Methods:

  • Review of recent literature on mass spectrometry techniques for single-cell proteomics.
  • Analysis of current challenges and limitations.
  • Exploration of future opportunities and applications.

Main Results:

  • Mass spectrometry-based single-cell proteomics is rapidly developing.
  • This approach offers a more comprehensive view of cellular functions compared to transcriptomics.
  • Significant challenges remain in sensitivity, throughput, and data analysis.

Conclusions:

  • Mass spectrometry-based single-cell proteomics is a promising technology.
  • It has the potential to revolutionize our understanding of complex biological systems.
  • Further technological development is needed to fully realize its potential in biomedical research.