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Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
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Protein Complex Assembly02:41

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
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Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
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Related Experiment Video

Updated: Sep 21, 2025

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
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Diazepinone HBV capsid assembly modulators.

Scott D Kuduk1, Lindsey G DeRatt1, Bart Stoops2

  • 1Janssen Research and Development, 1400 McKean Road, Spring House, PA 19477, United States.

Bioorganic & Medicinal Chemistry Letters
|June 1, 2022
PubMed
Summary
This summary is machine-generated.

Researchers optimized diazepinone compounds to target the Hepatitis B virus (HBV) capsid core protein, a key factor in persistent HBV infection. These optimized capsid assembly modulators (CAMs) show promise for developing new antiviral therapies.

Keywords:
CapsidDiazepinoneHBV

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Area of Science:

  • Hepatology
  • Virology
  • Medicinal Chemistry

Background:

  • The Hepatitis B virus (HBV) capsid core protein is crucial for viral replication and persistence, making it a significant drug target.
  • Small molecule capsid assembly modulators (CAMs) have demonstrated clinical efficacy by interfering with HBV capsid formation.

Purpose of the Study:

  • To further optimize a series of diazepinone-based HBV CAMs.
  • To enhance the therapeutic potential of compounds targeting HBV capsid assembly.

Main Methods:

  • Systematic chemical modification and structure-activity relationship (SAR) studies of diazepinone derivatives.
  • In vitro assays to evaluate antiviral activity and mechanism of action.

Main Results:

  • Identification of optimized diazepinone HBV CAMs with improved properties.
  • Demonstration of enhanced interference with HBV capsid assembly and viral replication.

Conclusions:

  • The optimized diazepinone HBV CAMs represent a promising advancement in the development of novel antiviral agents against chronic Hepatitis B infection.
  • Further development of these compounds could lead to effective treatments for HBV.