Related Experiment Video
Updated: Sep 21, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
Published on: July 27, 2022
Bleaching effect of violet LED of 405-410 nm on stained endodontically treated teeth
Leonardo Moreira Teodosio1, Leticia Gambarini1, André Luis Faria-E-Silva2
1Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, Western Subsector, 11 (N-11), Ribeirão Preto, SP 14040-904, Brazil.
Aim:
To evaluate in vitro the whiteness index (WID) and the whiteness index variation (ΔWID), as well as the color stability of stained endodontically treated teeth after bleaching with violet LED (VL) and with hydrogen peroxide 35% (HP) associated or not to VL.
Methods:
Twenty-four lower incisors were selected. The color was measured using a spectrophotometer at the following times: baseline, after staining, bleaching, and thermocycling, to determine the whiteness index and variation (WID and ΔWID). For the staining process, the teeth were immersed in human blood and centrifuged. After biomechanical preparation, the specimens were distributed into three groups (n = 8): control group (HP); violet LED (VL); and HP 35% + VL (HP+VL), and one session was performed for both bleaching procedures every week for three weeks, followed by thermocycling, where 10,000 cycles were performed. After color analysis, the data obtained were submitted to statistical analysis (RM ANOVA, p < 0,05).
Results:
The staining of the teeth reduced the WID values for all experimental groups compared to baseline, and the bleaching protocols increased WID values, with the lowest WID values for group VL. There was no statistically significant difference after thermocycling for all groups compared to bleaching. Staining and thermocycling reduced the ΔWID values, and no difference was found between treatments at these assessment times. Bleaching increased the ΔWID values, with less pronounced changes in the VL group.
Conclusion:
VL used alone has a lower bleaching effect compared to the bleaching treatment with HP, whether or not associated with VL, and the color stability was observed for all groups.
More Related Videos
05:12An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm
Published on: April 16, 2018
09:29In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging
Published on: April 28, 2017
Related Concept Videos
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...