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Updated: Sep 21, 2025
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
[Study of three kinds of primary immunization schedules with poliovirus vaccine]
Insights
Comparing poliovirus vaccine schedules, this study found all three regimens induced high antibody levels in infants. The 2sIPV+1bOPP and 3sIPV schedules showed better immunogenicity against type II poliovirus than 1sIPV+2bOPV.
Area of Science:
- Immunology
- Vaccinology
- Virology
Background:
- Poliovirus remains a global health concern, necessitating effective vaccination strategies.
- Understanding the immunogenicity of different poliovirus vaccine schedules is crucial for optimizing public health interventions.
Purpose of the Study:
- To compare the immunogenicity of three distinct poliovirus vaccine immunization programs in infants.
- To evaluate the development of poliovirus neutralizing antibodies following different vaccination schedules.
Main Methods:
- Healthy infants were randomized into three groups receiving different combinations of inactivated poliovirus vaccine (sIPV) and bivalent oral poliovirus vaccine (bOPV).
- Serum samples were collected before and 28-42 days after basic immunization.
- Polio neutralizing antibody levels were measured using the microcell neutralization method and analyzed statistically.
Main Results:
- All three immunization programs achieved high positive conversion rates (>97%) and positive rates (>98%) for poliovirus types I, II, and III antibodies.
- Significant increases in geometric mean titers (GMT) of neutralizing antibodies were observed in all groups post-immunization (P<0.05).
- The 2sIPV+1bOPV and 3sIPV groups demonstrated superior GMT for type II poliovirus compared to the 1sIPV+2bOPV group.
Conclusions:
- All evaluated poliovirus vaccine schedules effectively induce high levels of neutralizing antibodies, forming a strong immune barrier against poliovirus.
- While all schedules showed good immunogenicity, specific differences were noted, with 2sIPV+1bOPV and 3sIPV offering better protection against type II poliovirus.
Abstract:
Objective: To compare the immunogenicity of three kinds immunization programs with poliovirus vaccine. Methods: Healthy infants aged 2 months or over were selected and divided into three groups by complete randomization method. Basic immunization with Sabin inactivated poliovirus vaccine(sIPV) and bivalent oral poliovirus vaccine(bOPV) were completed. Three kinds of basic immunization procedures were 1sIPV+2bOPV,2sIPV+1bOPV and 3sIPV, respectively.Two qualified serums that before basic immunization and 28-42 days later were collected, and measured the poliovirus neutralizing antibody with microcell neutralization method. To compare the difference by analysis of variance, rank test and χ2 test. Results: After the basic immunization, 205 subjects of the positive conversion rate of poliovirus neutralizing antibodies of types Ⅰ, Ⅱ and Ⅲwere all higher than 97.00%, and the positive rates were all higher than 98.00%, the geometric mean titer (GMT) of neutralizing antibody was significantly higher than that before basic immunization in three groups.There were significant differences in the positive rate and GMT before and after basic immunization of typeⅠ, Ⅱand Ⅲ in the three (P<0.05). The highest GMT in three groups after basic immunization were all typeⅠ, followed by type Ⅲ, and the lowest in type Ⅱ. The GMT of type Ⅱin 2sIPV+1bOPV and 3sIPV groups were both higher than that in sIPV+2bOPV group. Conclution: After three kinds of basic immunization, the poliovirus neutralizing antibodies of serum were all at high levels in three groups, which could form an effective immune barrier against poliovirus. The immunogenicity of three kinds of basic immunization programs were all well, but there were certain differences of neutralizing antibodies among three kinds basic immunization programs. The immunogenicity in 2sIPV+1bOPV and 3sIPV groups against typeⅡpoliovirus were better than that in 1sIPV+2bOPV group.
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