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A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis

Teruo Akuta1, Toshiaki Maruyama2, Chiaki Sakuma1

  • 1Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna, Takahagi-shi 318-0004, Ibaraki, Japan.

Antibodies (Basel, Switzerland)
|June 1, 2022
PubMed

In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescent protein showed functional state both on agarose gel and blotted membrane. Based on the combined procedures, we discovered conformation-specific monoclonal antibodies against PLXDC2 and SARS-CoV-2 spike protein.

Keywords:
SARS-CoV-2antibodyblottingconformation-indifferentialconformation-specificnative gel

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