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A Novel Karyoplasmic Ratio-Based Automatic Recognition Method for Identifying Glioma Circulating Tumor Cells
Xinyi Zhu1, Shen Wen2, Shuhang Deng1
1Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China.
Frontiers in Oncology
|June 1, 2022
Summary
We developed a new method using karyoplasmic ratio (KR) to efficiently detect glioma circulating tumor cells (CTCs). This approach correlates CTC counts with patient characteristics, improving diagnosis and potentially reducing costs.
Area of Science:
- Oncology
- Biotechnology
- Medical Diagnostics
Background:
- Circulating tumor cells (CTCs) detection is crucial for tumor management but faces challenges in identification efficiency and clinical utility.
- Current methods for CTC identification are slow and complex, hindering widespread clinical application.
- Glioma CTC detection requires improved methods for accurate isolation and identification.
Purpose of the Study:
- To develop an efficient method for identifying glioma CTCs using a karyoplasmic ratio (KR)-based approach.
- To construct an automatic recognition algorithm for glioma CTCs.
- To investigate the correlation between high-KR CTCs and patients' clinical characteristics.
Main Methods:
- CTCs were isolated from 68 glioma patients' peripheral blood samples.
- Analysis involved DNA-seq, immunofluorescence staining, and ROC curve evaluation for the KR-based method.
- An automatic CTC recognition algorithm was developed using captured CTC images.
Main Results:
- The karyoplasmic ratio (KR) proved more effective than cell size for identifying glioma CTCs.
- Low CTC counts were independently associated with IDH mutations and 1p19q co-deletion, predicting oligodendroglioma (AUC=0.770).
- The developed algorithm achieved 93.4% accuracy, 81.0% sensitivity, and 97.4% specificity.
Conclusions:
- The study significantly enhanced glioma CTC detection efficiency and revealed correlations with clinical characteristics.
- The automatic recognition algorithm offers high precision, reduced time and cost, and less burden on clinicians.
- Findings support further investigation into the clinical utility of CTCs in glioma management.
