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Updated: Sep 21, 2025

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

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Zombies Never Die: The Double Life Bub1 Lives in Mitosis

Yuqing Zhang1, Chunlin Song1, Lei Wang1

  • 1The Cancer Institute, The Affiliated Hospital of Qingdao University, Qingdao, China.

Frontiers in Cell and Developmental Biology
|June 1, 2022
PubMed

Insights

Bub1 kinase is crucial for accurate chromosome segregation and cell cycle regulation during mitosis. This review details Bub1

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Mitosis requires precise chromosome alignment and segregation for accurate cell division.
  • The spindle assembly checkpoint (SAC) prevents premature anaphase onset until all chromosomes are properly attached.
  • Bub1 kinase is a key regulator of both chromosome alignment and the SAC during mitosis.

Purpose of the Study:

  • To review the dual roles of Bub1 kinase in supporting chromosome alignment and the spindle assembly checkpoint.
  • To discuss the essentiality of Bub1 in higher eukaryotic cells.
  • To highlight unresolved questions for future research.

Main Methods:

  • Literature review of studies on Bub1 function in mitosis.
  • Analysis of experimental data on Bub1's role in chromosome segregation and checkpoint control.
  • Comparative discussion of Bub1's essentiality across different eukaryotic species.

Main Results:

  • Bub1 kinase plays critical roles in kinetochore-microtubule attachments and chromosome congression to the metaphase plate.
  • Bub1 is essential for activating the spindle assembly checkpoint, ensuring proper chromosome segregation.
  • Emerging evidence highlights Bub1's multifaceted functions beyond checkpoint control.

Conclusions:

  • Bub1 kinase is indispensable for accurate chromosome segregation and mitotic progression in higher eukaryotes.
  • Further investigation is needed to fully elucidate Bub1's mechanisms and essentiality.
  • Understanding Bub1's roles is crucial for addressing aneuploidy and related disorders.
Keywords:
Bub1chromosome alignmentkinetochoremitosisspindle assembly checkpoint

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