Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies for Cardiovascular System V: CT01:28

Imaging Studies for Cardiovascular System V: CT

75
Cardiac computed tomography (CT) scanning is an advanced cardiac imaging technique that utilizes CT technology, with or without intravenous (IV) contrast, to produce accurate cross-sectional virtual slices of specific areas of the heart, coronary circulation, and major blood vessels such as the aorta, pulmonary veins, and arteries. The computer processes these slices to generate three-dimensional images. Multidetector CT (MDCT) is a rapid form of CT scanning that captures multiple slices...
75

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computer-assisted surgical navigation opens new horizons in veterinary surgery.

American journal of veterinary research·2026
Same author

PREVALENCE OF DIGITAL DERMATITIS IN EUROPEAN BISON (<i>BISON BONASUS</i>) IN SWITZERLAND AND REFERENCE TO OTHER EUROPEAN COUNTRIES.

Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians·2026
Same author

Comments on 'Spontaneous regression of equine sarcoids is an exceptional event'.

Equine veterinary journal·2026
Same author

First Complete Morphological Description of Schistosoma Reflexum in a Llama.

Veterinary medicine and science·2026
Same author

Case Report: Cervical internal vertebral venous plexus thrombosis diagnosed using time-of-flight magnetic resonance angiography in a dog.

Frontiers in veterinary science·2026
Same author

Comparison of Ultrasonography, Contrast Radiographic Tenography, Cone-Beam Computed Tomographic Tenography, and Tenoscopy for Lesion Detection Within the Digital Flexor Tendon Sheath of Horses-A Prospective Clinical Trial.

Veterinary sciences·2026

Related Experiment Video

Updated: Sep 21, 2025

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
04:35

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Published on: October 7, 2021

2.5K

Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study.

Chantal Blaettler1, Sabine Kaessmeyer2, Silke Grabherr3

  • 1Division of Clinical Radiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Frontiers in Veterinary Science
|June 1, 2022
PubMed
Summary

This study shows that perfusing frozen equine limbs with lipophilic contrast agents allows for high-quality 3D vascular imaging. This technique provides a feasible model for evaluating equine limb vasculature in situ.

Keywords:
PMCTAcadaverhorseperfusionvascular anatomy

More Related Videos

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of &#946;-aminopropionitrile-induced Aortic Aneurysm and Dissection
06:46

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of β-aminopropionitrile-induced Aortic Aneurysm and Dissection

Published on: July 16, 2018

9.7K
High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model
08:16

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

Published on: March 16, 2022

3.6K

Related Experiment Videos

Last Updated: Sep 21, 2025

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
04:35

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Published on: October 7, 2021

2.5K
Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of &#946;-aminopropionitrile-induced Aortic Aneurysm and Dissection
06:46

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of β-aminopropionitrile-induced Aortic Aneurysm and Dissection

Published on: July 16, 2018

9.7K
High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model
08:16

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

Published on: March 16, 2022

3.6K

Area of Science:

  • Veterinary Anatomy
  • Medical Imaging
  • Equine Medicine

Background:

  • Understanding equine distal limb vasculature is crucial for diagnosing and treating diseases.
  • High-resolution 3D visualization models are needed for better insights into vascular pathophysiology.
  • Current methods may lack the detail required for comprehensive vascular assessment.

Purpose of the Study:

  • To evaluate the feasibility of restoring vascular perfusion in frozen-thawed equine distal limbs.
  • To assess the quality of 3D vascular visualization using computer tomographic imaging (CT) after perfusion.
  • To compare the effectiveness of lipophilic versus hydrosoluble contrast agents for equine limb vascular imaging.

Main Methods:

  • Five frozen-thawed equine forelimbs were perfused with a lipophilic contrast agent via median artery and radial vein.
  • Perfusion occurred in three dynamic phases: arterial, venous, and arterial-venous combined (AVC).
  • CT scans were analyzed for vascular visualization, artifacts, and anatomical variations across 11 regions; one limb used hydrosoluble contrast for comparison.

Main Results:

  • The lipophilic contrast agent protocol demonstrated feasibility, achieving 93.1% vascular visualization with low artifact rates.
  • Vascular visualization quality was similar across perfusion phases, with decreasing filling defects and increasing extravasation in later phases.
  • The arterial-venous combined (AVC) dynamic phase subjectively yielded the best angiographic image quality.
  • Hydrosoluble contrast resulted in significantly lower visualization (74.0%) and higher artifact rates.

Conclusions:

  • Reperfusion of frozen-thawed equine distal limbs with lipophilic contrast agents enables high-quality 3D vascular visualization.
  • This method is feasible and offers a promising model for future in situ vascular evaluation in equine limbs.
  • Lipophilic contrast agents are superior to hydrosoluble agents for this specific application, providing better detail and fewer artifacts.