Nocardioides panacis sp. nov., isolated from soil of a ginseng field

A bacterial strain designated as G188^T was isolated from ginseng field soil in the Republic of Korea. Phylogenetic analysis of 16S rRNA gene sequences showed that strain G188^T formed a distinct lineage within the genus Nocardioides, family Nocardioidaceae, order Propionibacteriales. Sequence similarity revealed that strain G188^T was most closely related to Nocardioides iriomotensis IR27-S3^T (97.7 % 16S rRNA similarity). The genome size of strain G188^T was 4 901 775 bp, and the genomic DNA G+C content was 72.3 mol%. The average nucleotide identity and DNA-DNA hybridization values with other Nocardioides species were less than 75.6 and 20.1 %, respectively. The main fatty acids of strain G188^T were C_17 : 0, C_17 : 1  ω8c and iso-C_16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol, and the major respiratory quinone was menaquinone 8, supporting that strain G188^T was affiliated with the genus Nocardioides. Based on biochemical, chemotaxonomic and phylogenetic analyses, the novel species Nocardioides panacis G188^T (KACC 21695^T=LMG 31733^T) is proposed.