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Updated: Sep 21, 2025

Generation of Alginate Microspheres for Biomedical Applications
10:33

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Alginate Particles for Enzyme Immobilization Using Spray Drying.

Yilun Weng1, Supun Ranaweera2, Da Zou1,3

  • 1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.

Journal of Agricultural and Food Chemistry
|June 1, 2022
PubMed
Summary
This summary is machine-generated.

Spray drying enzyme immobilization using alginate particles significantly enhances phytase thermal stability. Immobilized phytase retains activity after heat exposure and in simulated digestion, showing industrial application potential.

Keywords:
alginatecontrolled releaseenzyme deliveryenzyme immobilizationparticle propertiesspray dryingthermal stability

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Area of Science:

  • Biocatalysis
  • Enzyme Engineering
  • Materials Science

Background:

  • Enzymes offer high catalytic efficiency but suffer from limited thermal stability, hindering industrial use.
  • Enzyme immobilization is a key strategy to improve stability and reusability.
  • Phytase is crucial in animal feed for phosphorus utilization.

Purpose of the Study:

  • To develop a scalable spray-drying method for enzyme immobilization.
  • To enhance the thermal stability of phytase using alginate particles.
  • To evaluate the functional recovery of immobilized phytase.

Main Methods:

  • Enzyme immobilization of phytase using alginate via spray drying.
  • Characterization of alginate particle physicochemical properties (size, morphology, heat resistance).
  • Confocal microscopy for immobilization confirmation and activity assays under thermal stress and simulated digestion.

Main Results:

  • Spray-dried alginate particles successfully immobilized phytase.
  • Immobilized phytase retained 58% activity after heating at 95°C, versus 4% for free enzyme.
  • Immobilized phytase showed >95% activity recovery after simulated gastrointestinal tract incubation.

Conclusions:

  • Spray drying with alginate is an effective and scalable method for enhancing enzyme thermal stability.
  • Immobilized phytase demonstrates improved heat resistance and functional recovery, suitable for industrial applications.
  • This approach is applicable to other enzymes for diverse biotechnological uses.