Controlling SpyTag/SpyCatcher Reactivity via Redox-Gated Conformational Restriction
Wen-Hao Wu1, Jingjing Wei2, Wen-Bin Zhang1
1Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
Abstract:
Herein, we report that the reactivity of genetically encoded SpyTag/SpyCatcher chemistry can be manipulated via redox-gated conformational restriction, which facilitates the preparation of all-protein-based hydrogel with latent reactive sites for subsequent covalent functionalization.
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