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Related Experiment Video

Updated: Sep 21, 2025

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
08:04

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

26.8K

Filtration extraction method using a microfluidic channel for measuring environmental DNA.

Takashi Fukuzawa1,2, Yuichi Kameda1,3, Hisao Nagata1

  • 1Go!Foton Inc., Tsukuba, Japan.

Molecular Ecology Resources
|June 2, 2022
PubMed
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The new Biryu-Chip (BC) method enables rapid, on-site environmental DNA (eDNA) analysis using minimal sample volumes. This microfluidic approach streamlines eDNA extraction and PCR, offering comparable results to traditional lab methods.

Area of Science:

  • Environmental science
  • Molecular biology
  • Biomonitoring technology

Background:

  • Environmental DNA (eDNA) analysis is crucial for biomonitoring but traditionally requires extensive laboratory processing.
  • Current eDNA methods often necessitate large sample volumes, limiting field applicability and efficiency.

Purpose of the Study:

  • To develop a microfluidic filtration/extraction component, the Biryu-Chip (BC), and associated method.
  • To reduce the sample volume required for eDNA extraction and subsequent PCR amplification.
  • To enable rapid, on-site eDNA analysis.

Main Methods:

  • Development of the Biryu-Chip (BC) microfluidic device for filtration and extraction.
  • Comparison of the BC method with the Sterivex filtration/extraction method using aquarium and river water samples.
Keywords:
eDNAextractionfilteringmicrofluidic

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Related Experiment Videos

Last Updated: Sep 21, 2025

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

26.8K
Air-sampled Filter Analysis for Endotoxins and DNA Content
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  • On-site eDNA measurements using the BC method combined with a mobile PCR device.

    • Main Results:

    • The BC method achieved equivalent DNA concentrations with 1/20-1/40 of the filtration volume compared to the Sterivex method.
    • On-site eDNA measurements were successfully performed within 30 minutes using the BC method and mobile PCR.
    • PCR results from the BC method were comparable to those from the Sterivex method.
    • The BC method involves fewer steps, reducing the risk of DNA contamination.

    Conclusions:

    • The BC method offers a highly efficient and rapid approach for eDNA analysis, significantly reducing sample volume requirements.
    • The combination of the BC method with mobile PCR facilitates quick, on-site eDNA detection, broadening the scope of biomonitoring applications.
    • This microfluidic technology enhances the practicality and accessibility of eDNA measurements in diverse environmental settings.