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Updated: Sep 21, 2025
Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using 129Xe MRI
Published on: November 21, 2023
Quantitative assessment of regional lung ventilation in emphysematous mice using hyperpolarized 129Xe MRI with a
Tetsuya Wakayama1, Tsuyoshi Ueyama1, Fumito Imai1
1Department of Medical Physics and Engineering, Area of Medical Imaging Technology and Science, Division of Health Sciences, Graduate of School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
Background:
Lung ventilation function in small animals can be assessed by using hyperpolarized gas MRI. For these experiments a free breathing protocol is generally preferred to mechanical ventilation as mechanical ventilation can often lead to ventilation lung injury, while the need to maintain a gas reservoir may lead to a partial reduction of the polarization.
Purpose:
To evaluate regional lung ventilation of mice by a simple but fast method under free breathing and give evidence for effectiveness with an elastase instilled emphysematous mice.
Animal Model:
Emphysematous mice.
Materials And Methods:
A Look-Locker based saturation recovery sequence was developed for continuous flow hyperpolarized (CF-HP) 129Xe gas experiments, and the apparent gas-exchange rate, k', was measured by the analysis of the saturation recovery curve.
Results:
In mice with elastase-induced mild emphysema, reductions of 15-30% in k' values were observed as the results of lesion-induced changes in the lung.
Data Conclusion:
The proposed method was applied to an emphysematous model mice and ventilation dysfunctions have been approved as a definite decrease in k' values, supporting the usefulness for a non-invasive assessment of the lung functions in preclinical study by the CF-HP 129Xe experiments.
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