Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HPV18 oncogenes increase viability and nuclear architecture stability in HaCaT cells exposed to UVB radiation.

Tissue & cell·2026
Same author

Bandwidth-optimized regularization for low-frequency recovery in quantitative phase imaging.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same author

Rare variants in embryonic development and cell signalling genes in syndromic and non-syndromic orofacial clefts: evidence from a Colombian Caribbean cohort.

Journal of human genetics·2026
Same author

Fostering Motivation for Practice Change: Using Self-Determination Theory to Guide Mental Health Training.

Community mental health journal·2026
Same author

GPU-optimized matrix method for phase retrieval with the transport of intensity equation.

Applied optics·2025
Same author

Quantitative phase microscopy for time-lapse hypoxia-induced cellular assays based on the transport of intensity equation.

Applied optics·2025

Related Experiment Video

Updated: Sep 21, 2025

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.2K

Computational multifocus fluorescence microscopy for three-dimensional visualization of multicellular tumor

Julia R Alonso1, Alejandro Silva1, Ariel Fernández1

  • 1Universidad de la República, Instituto de Física, Facultad de Ingeniería, Montevideo, Uruguay.

Journal of Biomedical Optics
|June 3, 2022
PubMed
Summary

This study presents a computational multifocus fluorescence microscopy method for 3D visualization of multicellular tumor spheroids (MCTS). The technique overcomes depth-of-field limitations, enabling detailed morphological assessment of cellular aggregates.

Keywords:
computational optical imagingfluorescence microscopystereoscopic pairsthree-dimensional visualization

More Related Videos

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model
07:42

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Published on: January 30, 2021

6.6K
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.8K

Related Experiment Videos

Last Updated: Sep 21, 2025

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.2K
Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model
07:42

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Published on: January 30, 2021

6.6K
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.8K

Area of Science:

  • Biomedical Imaging
  • Cell Biology
  • Microscopy

Background:

  • Three-dimensional (3D) visualization of multicellular tumor spheroids (MCTS) in fluorescence microscopy offers rapid qualitative morphological insights into cellular aggregate architecture.
  • MCTS recapitulate key aspects of in vivo tumor structures, making them valuable models for cancer research.

Purpose of the Study:

  • To overcome the shallow depth-of-field (DoF) limitation in fluorescence microscopy for 3D visualization of thick biological samples.
  • To enable detailed 3D morphological analysis of multicellular tumor spheroids.

Main Methods:

  • Development of a custom-built fluorescence microscope equipped with an electrically focus-tunable lens.
  • Optical in-depth sweeping of MCTS structures and acquisition of multifocus image stacks.
  • Postprocessing of image stacks using Fourier domain algorithms to combine data.

Main Results:

  • Achieved extended DoF, stereoscopic pairs, and reconstructed viewpoints of MCTS without image segmentation or depth map estimation.
  • Successfully visualized the 3D morphology of cell aggregates.
  • Obtained high-quality 3D images preserving relevant morphological characteristics.

Conclusions:

  • Computational multifocus fluorescence microscopy provides effective 3D visualization of MCTS.
  • This technique is a promising tool for assessing the morphological structure of cellular aggregates.
  • The developed optical setup is robust yet simple, facilitating broader application.