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Microsphere reference flow samples during systemic flow adjustment.

G A Geffin, D D O'Keefe, A G Denenberg

    The American Journal of Physiology
    |April 1, 1987
    PubMed
    Summary
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    Accurate myocardial blood flow measurement is crucial. This study found aortic reference samples are valid for coronary blood flow assessment in right heart bypass preparations under specific hemodynamic conditions, ensuring reliable research data.

    Area of Science:

    • Cardiovascular Physiology
    • Hemodynamics
    • Experimental Medicine

    Background:

    • Regional myocardial blood flow (MBF) is critical for assessing cardiac health.
    • The right heart bypass preparation offers controlled hemodynamic conditions for MBF studies.
    • Accurate reference flow sampling is essential for valid MBF measurements.

    Purpose of the Study:

    • To validate the use of aortic reference flow samples for measuring coronary blood flow.
    • To assess the accuracy of microsphere concentration in aortic versus coronary samples.
    • To determine the optimal hemodynamic conditions for valid reference sampling in a right heart bypass model.

    Main Methods:

    • Six dogs were studied using a right heart bypass preparation under anesthesia.

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  • Microsphere concentrations were compared between aortic arch and coronary artery samples.
  • Systemic flow was continuously adjusted to control aortic pressure.
  • Paired samples were analyzed across various infusion rates and cardiac outputs.

    • Main Results:

    • High percentage errors in microsphere concentration occurred with high-volume systemic artery infusions (>2,000 ml/min).
    • Under specific conditions (withdrawal or low-rate femoral infusion, <2,000 ml/min), percentage error was minimal (0.001 +/- 1.18% SE).
    • Error was <20% in 96% and <10% in 77% of valid injections.
    • A strong linear correlation (r=0.997) was found between coronary and aortic microsphere concentrations.

    Conclusions:

    • Aortic reference flow samples are valid for MBF measurements in right heart bypass preparations.
    • Validity is dependent on specific hemodynamic conditions, particularly avoiding high-volume systemic infusion.
    • These findings support the use of aortic samples for accurate coronary blood flow quantification in this experimental model.