Circulating S-Glutathionylated cMyBP-C as a Biomarker for Cardiac Diastolic Dysfunction

Area of Science:

• Cardiology
• Biochemistry
• Biomarker Discovery

Background:

• Cardiac myosin binding protein-C (cMyBP-C) is crucial for regulating heart muscle contraction and relaxation.
• Elevated S-glutathionylation of cMyBP-C in the heart muscle is linked to diastolic dysfunction (DD) in animal models.

Purpose of the Study:

• To investigate if circulating S-glutathionylated cMyBP-C can serve as a biomarker for diastolic dysfunction (DD).

Main Methods:

• Diastolic dysfunction (DD) was assessed using echocardiography in humans, African Green monkeys, and mice.
• Circulating levels of S-glutathionylated cMyBP-C were measured via immunoprecipitation in blood samples.

Main Results:

• Humans, monkeys, and mice with DD exhibited significantly elevated levels of circulating S-glutathionylated cMyBP-C compared to controls.
• A positive correlation was observed between circulating S-glutathionylated cMyBP-C and DD in human participants.

Conclusions:

• Circulating S-glutathionylated cMyBP-C is elevated in individuals with DD across multiple species.
• S-glutathionylated cMyBP-C shows potential as a novel biomarker for the presence of diastolic dysfunction.