Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Activation of Integrins01:15

Activation of Integrins

3.6K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
3.6K
Integrins01:10

Integrins

4.2K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
4.2K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

7.4K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.4K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.8K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.8K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.3K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor.

PLoS biology·2025
Same author

Small but mighty: ATG9A-positive vesicles are a branch of the intracellular nanovesicle superfamily.

Autophagy reports·2025
Same author

Structural characterization and inhibition of the interaction between ch-TOG and TACC3.

The Journal of cell biology·2025
Same author

ATG9A vesicles are a subtype of intracellular nanovesicle.

Journal of cell science·2025
Same author

The Company of Biologists: celebrating 100 years.

Disease models & mechanisms·2025
Same author

The Company of Biologists: celebrating 100 years.

The Journal of experimental biology·2025

Related Experiment Video

Updated: Sep 21, 2025

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation
04:15

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

Published on: February 2, 2024

2.0K

Integrating intracellular nanovesicles into integrin trafficking pathways and beyond.

Gabrielle Larocque1, Stephen J Royle2

  • 1The Francis Crick Institute, Midland Road, London, NW1 1AT, UK.

Cellular and Molecular Life Sciences : CMLS
|June 3, 2022
PubMed
Summary

Intracellular nanovesicles (INVs) are small transport vesicles involved in cell functions like migration. This review details their role in membrane trafficking, particularly integrin recycling, and outlines future research directions.

Keywords:
IntegrinsIntracellular nanovesiclesMembrane traffickingMigrationRab30TPD54

More Related Videos

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.3K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.1K

Related Experiment Videos

Last Updated: Sep 21, 2025

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation
04:15

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

Published on: February 2, 2024

2.0K
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.3K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.1K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Membrane traffic is essential for cellular function, utilizing transport vesicles to move proteins and lipids.
  • Intracellular nanovesicles (INVs) represent a recently identified category of transport vesicles.
  • INVs are small, versatile, and implicated in various trafficking events, including anterograde transport and endosomal recycling.

Purpose of the Study:

  • To review current knowledge on intracellular nanovesicles (INVs).
  • To explore the integration of INVs into established membrane trafficking pathways, using integrin recycling as a model.
  • To speculate on the cellular locations and functions of INVs and identify key areas for future research.

Main Methods:

  • Literature review and synthesis of existing research on INVs.
  • Analysis of the role of INVs in integrin recycling and cell migration.
  • Theoretical modeling and speculation on INV function and localization.

Main Results:

  • INVs are small vesicles involved in diverse trafficking steps, including anterograde traffic and endosomal recycling.
  • INVs play a role in cell migration and invasion through their involvement in integrin recycling.
  • The precise mechanisms and cellular locations of INVs are still under investigation.

Conclusions:

  • INVs are a significant component of cellular membrane trafficking systems.
  • Further research is needed to fully elucidate the functions and operational sites of INVs within the cell.
  • Understanding INVs could provide new insights into processes like cell migration and invasion.