Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Engineered clMagR Tetramer with Enhanced Magnetism for Magnetic Manipulation.

Biomolecules·2026
Same author

Structural Analysis of Human LonP1 Protease Bound with the Native Substrate.

Life (Basel, Switzerland)·2026
Same author

Interaction and Reciprocal Stabilization of H3.3 and PARP1 Promote DNA Damage Repair and Tumor Progression in Small Cell Lung Cancer.

Cancer research·2025
Same author

A Robust Immobilization Method for Membrane Protein SPR Assays Using SpyCatcher-SpyTag.

Analytical chemistry·2025
Same author

Efficient and Specific PDGFRβ-Targeting Dual-Mode T<sub>1</sub>-T<sub>2</sub> MRI Nanoprobe for Early Diagnosis of Non-Alcoholic Fatty Liver.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Backbone resonance assignments of PhoCl, a photocleavable protein.

Biomolecular NMR assignments·2025

Related Experiment Video

Updated: Sep 21, 2025

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

9.8K

Site-Specific 19F NMR Method for Detecting Arf6 GEF Activity.

Jiarong Wang1, Bo Wu1, Youjia Zhang1,2

  • 1High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China.

Analytical Chemistry
|June 6, 2022
PubMed
Summary

Researchers developed a novel 1D 19F NMR method to rapidly detect guanine nucleotide exchange factor (GEF) activity. This technique monitors conformational changes in small GTPases (sGTPases), aiding the study of cellular signaling and disease.

More Related Videos

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
09:24

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products

Published on: August 22, 2017

8.5K
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

19.0K

Related Experiment Videos

Last Updated: Sep 21, 2025

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

9.8K
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
09:24

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products

Published on: August 22, 2017

8.5K
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
12:47

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

19.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Guanine nucleotide exchange factors (GEFs) regulate small GTPase (sGTPase) signaling, which is crucial in normal cellular functions and implicated in cancer.
  • Dysfunctional sGTPase signaling contributes to various pathologies, highlighting the need for effective methods to monitor GEF activity.

Purpose of the Study:

  • To develop a rapid and convenient method for detecting GEF activity using 1D 19F NMR spectroscopy.
  • To enable the monitoring of sGTPase conformational changes indicative of GEF activity in various environments.

Main Methods:

  • Development of a 1D 19F NMR-based assay.
  • Site-specific 19F labeling of Arf6 at Phe47 to probe conformational changes in the switch region.
  • Detection of Arf6GEF activity in vitro and in cell lysates.

Main Results:

  • Successfully demonstrated the detection of Arf6GEF activity by monitoring conformational changes via 19F NMR.
  • The method allows for convenient and rapid assessment of GEF activity.
  • The introduced 19F label at Phe47 serves as a sensitive probe for Arf6 conformational dynamics.

Conclusions:

  • The developed 1D 19F NMR method provides a powerful tool for studying sGTPase regulation.
  • This strategy is applicable to monitoring conformational changes in Arf6 and potentially other sGTPases.
  • The technique can be utilized to detect the activity of sGTPase regulatory proteins in physiological and pathological contexts.