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Related Experiment Video

Updated: Sep 21, 2025

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

SEB genotyping: SmartAmp-Eprimer binary code genotyping for complex, highly variable targets applied to HBV.

Diane Delobel¹, Yutaka Furutani², Sumiko Nagoshi³

¹Genetic Diagnosis Technology Unit, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, Japan.

BMC Infectious Diseases

|June 6, 2022

Summary

SmartAmp-Eprimer Binary code (SEB) Genotyping offers rapid, accurate identification of viral genotypes. This novel isothermal method provides a unique binary code for each genotype, aiding in personalized treatment decisions.

Keywords:

Genotyping Hepatitis B virus High mutation rate Infectious diseases Isothermal amplification

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Last Updated: Sep 21, 2025

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Molecular Biology
Genetics
Virology

Background:

SmartAmp-Eprimer Binary code (SEB) Genotyping is a novel isothermal amplification technique.
It is designed for rapid genotyping of variable targets.

Purpose of the Study:

To present the proof of concept for SEB Genotyping.
To demonstrate its application in Hepatitis B Virus (HBV) genotyping.

Main Methods:

In silico sequence alignment and computational analysis identified key target regions.
SmartAmp primer sets were designed to generate On/Off fluorescence signals, creating a binary code for each genotype.

Main Results:

Applied to HBV, 4 targets were selected to generate a unique binary code for each of the 8 main genotypes (A-H).
The method demonstrated accurate results quickly.

Conclusions:

SEB Genotyping is a new method for complex, highly variable targets.
It can be adapted for genotyping other pathogens or diseases with multiple mutations.
The technique enables rapid, accurate results for informed treatment selection.