Application of Non-invasive Micro-test Technology (NMT) in environmental fields: A comprehensive review

Non-invasive Micro-test Technology (NMT) is a selective microelectrode technique which can detect the flux rates and three-dimensional motion directions of ions or molecules into and out of living organisms in situ without damaging the sample. It has the advantages of maintaining sample integrity, high temporal and spatial resolution, and being able to measure multiple sites simultaneously. In this paper we provide a comprehensive review on the development of NMT in recent years. Its principles, characteristics, and the differences with other microelectrode techniques are introduced. We discuss the applications of NMT in the field of phytoremediation, plant resistance, water quality monitoring, and toxicity mechanisms of heavy metals on organisms. Furthermore, the challenges and future prospects of NMT in the environmental field are presented.