MoS₂ quantum dots as fluorescent probe for methotrexate detection

Herein, we developed a unique fluorescence biosensor for methotrexate assay based on MoS₂ quantum dots, which were synthesized in one step using sodium molybdate and cysteine as raw materials. The fluorescence of MoS₂ QDs could be quenched when encountered with methotrexate, which was attributed to the inner filter effect (IFE). Furthermore, this present IFE-based method showed the linearity between the MoS₂ QDs fluorescence intensity and the methotrexate concentration in the range of 0.05-1 μM with the LOD of 42 nM. The practical applicability of this strategy was successfully demonstrated by detecting methotrexate in real samples. Results indicated that the proposed method could be a promising sensing platform for methotrexate analysis.