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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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MoS2 quantum dots as fluorescent probe for methotrexate detection.

Like Yang1, Jia Ge1, Demiao Ma1

  • 1College of Chemistry, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|June 6, 2022
PubMed
Summary
This summary is machine-generated.

A new fluorescence biosensor uses molybdenum disulfide (MoS2) quantum dots to detect methotrexate. This method offers a sensitive and practical approach for methotrexate analysis in real samples.

Keywords:
BiosensorFluorescenceInner filter effectMethotrexateMoS(2) quantum dots

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Methotrexate (MTX) is a crucial drug in cancer chemotherapy.
  • Accurate and sensitive detection of MTX is vital for therapeutic drug monitoring.
  • Existing detection methods may have limitations in sensitivity, cost, or complexity.

Purpose of the Study:

  • To develop a novel and sensitive fluorescence biosensor for methotrexate (MTX) detection.
  • To utilize molybdenum disulfide (MoS2) quantum dots (QDs) as a fluorescent probe.
  • To establish a cost-effective and efficient platform for MTX assay.

Main Methods:

  • Synthesis of MoS2 quantum dots (QDs) from sodium molybdate and cysteine in a single step.
  • Utilizing the inner filter effect (IFE) for fluorescence quenching of MoS2 QDs by MTX.
  • Establishing the linear relationship between fluorescence intensity and MTX concentration.

Main Results:

  • The MoS2 QDs exhibited fluorescence quenching in the presence of MTX due to the IFE.
  • A linear correlation was observed between MoS2 QDs fluorescence intensity and MTX concentration from 0.05 to 1 μM.
  • The limit of detection (LOD) for MTX was determined to be 42 nM.
  • The biosensor demonstrated successful application in detecting MTX in real samples.

Conclusions:

  • The developed MoS2 QD-based fluorescence biosensor is a sensitive and effective tool for MTX detection.
  • The IFE mechanism provides a reliable basis for the quantitative analysis of MTX.
  • This sensing platform shows significant promise for practical methotrexate analysis and therapeutic drug monitoring.