Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 21, 2025

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.8K

Deep Metagenomic Sequencing for Endophthalmitis Pathogen Detection Using a Nanopore Platform.

Liying Low1, Kenji Nakamichi2, Lakshmi Akileswaran2

  • 1From the Academic Unit of Ophthalmology, Institute of Inflammation and Ageing, University of Birmingham, United Kingdom (L.L, P.I.M, G.R.W, S.R); Birmingham and Midland Eye Centre, Sandwell and West Birmingham Hospitals NHS Trust, Birmingham, United Kingdom (L.L, G.M, P.I.M, S.R).

American Journal of Ophthalmology
|June 6, 2022
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safe use of fluorinated gases in vitreoretinal surgery: learning from patient safety incidents with expert panel recommendations from the British and Eire Association of Vitreoretinal Surgeons (BEAVRS).

The British journal of ophthalmology·2026
Same author

Automated Deep Learning Quantification of Avascular Area and Intravitreal Neovascularization in Retinal Flatmounts of Rodent Oxygen-Induced Retinopathy Models.

Translational vision science & technology·2026
Same author

Uses and Abuses of Big Data in Ophthalmology Research.

Ophthalmology·2026
Same author

Traumatic brain injury and late-life Alzheimer's disease neuropathology: Quantitative investigation in the Adult Changes in Thought study.

Journal of Alzheimer's disease : JAD·2026
Same author

Quantitative Swept-Source Optical Coherence Tomography Angiography Indicators of Neurovascular Dysfunction in Alzheimer Disease.

JAMA ophthalmology·2026
Same author

Age-related macular degeneration severity and risk of Alzheimer's disease in older adults in a prospective, community-based cohort study.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026

Whole genome sequencing (WGS) offers a sensitive alternative to traditional culture and 16S sequencing for identifying endophthalmitis pathogens. Nanopore sequencing, in particular, shows promise for cost-effective, point-of-care diagnostics.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Genomics

Background:

  • Endophthalmitis is a serious intraocular infection requiring prompt diagnosis.
  • Conventional culture methods can be slow and may fail to detect all causative pathogens.
  • Next-generation sequencing offers potential for rapid and comprehensive pathogen identification.

Purpose of the Study:

  • To assess the diagnostic utility of nanopore sequencing for identifying endophthalmitis pathogens.
  • To compare nanopore sequencing (16S rRNA and whole genome) with conventional culture and Illumina whole genome sequencing (WGS).

Main Methods:

  • A cross-sectional study involving patients with suspected endophthalmitis.
  • Intraocular vitreous biopsies were subjected to conventional culture, 16S rRNA nanopore sequencing, whole genome nanopore sequencing (WGS), and Illumina WGS.

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.9K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.9K

Related Experiment Videos

Last Updated: Sep 21, 2025

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.8K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.9K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.9K

Main Results:

  • Nanopore sequencing correctly identified all culture-positive organisms and detected pathogens in 40% of culture-negative cases.
  • Nanopore WGS identified bacteriophages in three samples, a capability not assessed in culture.
  • Genus-level agreement with culture was highest for Nanopore WGS (100%), followed by Illumina WGS (78%) and 16S Nanopore (75%).

Conclusions:

  • Whole genome sequencing (WGS) demonstrates superior sensitivity for pathogen detection in endophthalmitis compared to culture and 16S sequencing.
  • WGS can identify pathogens in culture-negative samples and detect additional microbial elements like bacteriophages.
  • Nanopore sequencing presents a viable, potentially cost-effective option for point-of-care endophthalmitis diagnostics.