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Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
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Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

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Bacteriostatic effects of phage F23s1 and its endolysin on Vibrio parahaemolyticus

Hai Xia1, Houji Yang1, Na Yan1

  • 1Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.

Journal of Basic Microbiology
|June 6, 2022
PubMed

Insights

A novel bacteriophage, F23s1, and its endolysin show promise for controlling drug-resistant Vibrio parahaemolyticus. This phage therapy approach offers a potential solution for food safety and public health concerns.

Area of Science:

  • Microbiology
  • Bacteriology
  • Food Safety

Background:

  • Vibrio parahaemolyticus is a significant foodborne pathogen.
  • Drug-resistant strains are increasingly prevalent, posing public health challenges.
  • Bacteriophage therapy presents a potential alternative to antibiotics.

Purpose of the Study:

  • To isolate and characterize a bacteriophage targeting Vibrio parahaemolyticus.
  • To evaluate the efficacy of the bacteriophage and its derived endolysin in controlling V. parahaemolyticus.
  • To assess the potential of phage F23s1 and its endolysin in food safety applications.

Main Methods:

  • Isolation and characterization of Vibrio parahaemolyticus-specific bacteriophage F23s1.
  • Genome sequencing of phage F23s1 and identification of endolysin gene ORF52.
  • Cloning and expression of the endolysin gene in Escherichia coli.
  • In vitro and in vivo efficacy testing against V. parahaemolyticus.

Main Results:

  • Phage F23s1 demonstrated activity across a wide pH and temperature range.
  • F23s1 effectively inhibited V. parahaemolyticus growth in shrimp.
  • The recombinant endolysin ORF52 exhibited significant lytic activity against drug-resistant V. parahaemolyticus and Salmonella strains.

Conclusions:

  • Phage F23s1 and its endolysin are effective against Vibrio parahaemolyticus.
  • These agents show potential for preventing and controlling V. parahaemolyticus contamination in food.
  • This research contributes to the development of novel strategies for combating foodborne pathogens.
Keywords:
Vibrio parahaemolyticusendolysinphageshrimp

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