Nonylphenol induces anxiety-like behavior in rats by regulating BDNF/TrkB/CREB signal network

This study aimed to verify whether chronic exposure to nonylphenol (NP) induces anxiety behavior in rats and explored NP's regulatory effect on the BDNF/TrkB/CREB signal network in vitro. Anxiety-like behavior was assessed by elevated plus-maze and light-dark box tests. The residence time in the closed arm increased with NP dose (4, 40 mg/kg) and exposure time (3 and 6 months) (P < 0.05). The hippocampal neurons in the medium dose (M-NP, 4 mg/kg) and high dose (H-NP, 40 mg/kg) groups showed disorderly arrangement, cell swelling, and nuclear pyknosis/necrosis. The protein/mRNA expressions of BDNF/TrkB/CREB in the H-NP group decreased, and the decrease was more significant at 6 months (P < 0.05). Both, NP exposure and BDNF knockdown, increase the number of apoptotic cells (P ＜0.001). NP downregulated the proteins/mRNA expressions of BDNF/TrkB/CREB, and the trend was consistent with the BDNF silence group. Chronic exposure to NP could induce anxiety-like behavior in rats and reduce the expression of key proteins/genes in the BDNF/TrkB/CREB signaling network.