Related Experiment Video
Updated: Sep 20, 2025

Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
Published on: May 5, 2021
Adiponectin Ameliorates Hyperglycemia-Induced Retinal Endothelial Dysfunction, Highlighting Pathways, Regulators, and
Sumbul Bushra1, Duaa W Al-Sadeq1, Redwana Bari1
1Department of Biomedical Sciences, College of Health Sciences, QU-Health, Qatar University, Doha, Qatar.
Adiponectin (APN) combats hyperglycemia's damaging effects on retinal cells, reducing oxidative stress and apoptosis. This study shows APN protects against diabetic retinopathy (DR) progression by improving endothelial function and barrier integrity.
Area of Science:
- Ophthalmology
- Endocrinology
- Molecular Biology
Background:
- Diabetic retinopathy (DR) pathophysiology is complex, with low adiponectin (APN) levels in type 2 diabetes linked to microvascular complications.
- The precise role of APN in DR development remains intricate and requires further elucidation.
Purpose of the Study:
- To investigate the impact of APN on diabetic retinopathy (DR) pathogenesis.
- To connect cellular and biological changes with the molecular pathways and regulators influenced by APN.
Main Methods:
- Human microvascular retinal endothelial cells (HMRECs) were exposed to high glucose (HG) and treated with APN.
- Assessed reactive oxidative stress (ROS), apoptosis, barrier function, and leukocyte migration.
- RT-PCR arrays and bioinformatic analysis identified gene expression profiles and signaling pathways.
Main Results:
- APN suppressed ROS production and apoptosis in HG-treated HMRECs.
- APN enhanced cell migration and barrier function in hyperglycemic conditions.
- Bioinformatic analysis revealed integrin, HMGB1, and p38 AMPK pathways are key, with STAT3, NFKB, and TNF as top regulators.
Conclusions:
- APN counteracts hyperglycemia at cellular and molecular levels, mitigating DR progression in vitro.
- APN ameliorates inflammation, oxidative stress, and endothelial dysfunction via specific causal networks and pathways.
More Related Videos
09:03Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
Published on: June 13, 2013
10:46Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
Published on: October 7, 2018
Related Concept Videos
Pathophysiology of Diabetes
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
Insulin: The Receptor and Signaling Pathways
Dipeptidyl Peptidase 4 Inhibitors
Diabetes Mellitus: Type 2 and Gestational
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are...