Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glutamine enhances endothelial cell survival and vasodilation by increasing glutathione to reduce oxidative stress.

Physiological reports·2026
Same author

Cholesterol Depletion with U18666A and Methyl-β Cyclodextrin Increased Small Molecule Permeability Across Brain Microvascular Endothelial Cells.

Annals of biomedical engineering·2025
Same author

Piezo1 activates nitric oxide synthase in red blood cells via protein kinase C with increased activity in diabetes.

Mechanobiology in medicine·2025
Same author

Human Pulmonary Artery Endothelial Cells Increased Glycolysis and Decreased Nitric Oxide Synthase O-GlcNAcylation in Pulmonary Arterial Hypertension.

International journal of translational medicine (Basel, Switzerland)·2025
Same author

Glutamine metabolism is systemically different between primary and induced pluripotent stem cell-derived brain microvascular endothelial cells.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2025
Same author

Mechanisms of postischemic cardiac death and protection following myocardial injury.

The Journal of clinical investigation·2025

Related Experiment Video

Updated: Sep 20, 2025

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.2K

A simple method to align cells on 3D hydrogels using 3D printed molds.

Jesse Vo1, Yusuf Mastoor1, Pattie S Mathieu1

  • 1Fischell Department of Bioengineering University of Maryland 8278 Paint Branch Drive College Park, MD 20742, USA.

Biomedical Engineering Advances
|June 6, 2022
PubMed
Summary
This summary is machine-generated.

3D printed molds create topographical patterns on substrates to align vascular smooth muscle cells. This method effectively guides cell alignment on both stiff and soft 3D materials for tissue engineering applications.

Keywords:
3D printingCell alignmentHydrogel patterningVascular smooth muscle cells

More Related Videos

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
10:25

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Published on: December 21, 2019

19.0K
Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations
05:22

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Published on: March 21, 2019

5.8K

Related Experiment Videos

Last Updated: Sep 20, 2025

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.2K
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
10:25

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Published on: December 21, 2019

19.0K
Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations
05:22

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Published on: March 21, 2019

5.8K

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Vascular smooth muscle cells (VSMCs) require circumferential alignment for blood vessel function.
  • Existing cell alignment methods are limited, especially on 3D hydrogel substrates.
  • Tissue-engineered blood vessels necessitate recapitulating native VSMC alignment.

Purpose of the Study:

  • To investigate the use of 3D printed molds for topographical patterning of substrates.
  • To align vascular smooth muscle cells on both stiff and soft 3D materials.
  • To assess the efficacy of 3D printed molds in creating physiologically relevant cell alignment.

Main Methods:

  • Fused deposition modeling (FDM) 3D printing was used to create molds with varying ridge sizes (150, 250, 350 μm).
  • These molds topographically patterned polydimethylsiloxane (PDMS) and gelatin-methacryloyl (GelMA) substrates.
  • Vascular smooth muscle cells were seeded onto patterned substrates, and nuclear and actin alignment were quantified.

Main Results:

  • 3D printed ridges successfully transferred to both PDMS and GelMA substrates with minimal dimensional change (<10%).
  • Vascular smooth muscle cells exhibited high alignment with the topographical patterns on both substrate types.
  • Cell alignment was comparable on both stiff PDMS and soft GelMA hydrogels.

Conclusions:

  • FDM 3D printed molds offer a rapid and cost-effective method for topographical patterning of diverse 3D substrates.
  • This technique enables precise control over cell alignment on both polymeric and hydrogel materials.
  • The developed method facilitates cell patterning in 3D structures for advanced tissue engineering applications.