Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine learning risk stratification to identify people living with HIV at high risk of delayed ART and advanced immunosuppression: a precision public health approach.

Frontiers in public health·2026
Same author

Predicting immune reconstitution after antiretroviral therapy in HIV/AIDS using ensemble machine learning: a real-world study.

Frontiers in immunology·2026
Same author

Urinary Cytokines in Predicting Intradetrusor Onabotulinumtoxin-A Response.

Neurourology and urodynamics·2026
Same author

Effect of rapid antiretroviral therapy initiation on loss to follow-up, mortality, and virologic failure among people with human immunodeficiency virus under the treat-all policy in China: analysis of routine data.

Frontiers in cellular and infection microbiology·2026
Same author

Effects of Two Anti-HIV-1 Regimens on Creatinine and Blood Lipids in Elderly People Living With HIV-1.

AIDS research and treatment·2026
Same author

To explore the relationship between physical activity and sleep quality of college students based on the mediating effect of stress and subjective well-being.

BMC psychology·2025

Related Experiment Video

Updated: Sep 20, 2025

Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock
16:31

Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock

Published on: June 6, 2011

25.0K

Inferring Tissue-Specific, TLR4-Dependent Type 17 Immune Interactions in Experimental Trauma/Hemorrhagic Shock and

Ashti M Shah1, Ruben Zamora1,2, Sebastian Korff1

  • 1Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.

Frontiers in Immunology
|June 6, 2022
PubMed
Summary

Trauma and resuscitation cause inflammation, partly via Toll-like Receptor 4 (TLR4). Removing TLR4 altered inflammatory mediator distribution and suggested TLR4 controls T helper 17 cell trafficking to tissues after injury.

Keywords:
Toll-like 4 receptorcomputational biologyhemorrhagic shockinflammationsystems biologytrauma

More Related Videos

Author Spotlight: Developing Innovative Therapeutic Strategies for Hemorrhagic Shock Research
08:14

Author Spotlight: Developing Innovative Therapeutic Strategies for Hemorrhagic Shock Research

Published on: March 22, 2024

1.6K
Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring
06:11

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

Published on: May 21, 2017

8.7K

Related Experiment Videos

Last Updated: Sep 20, 2025

Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock
16:31

Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock

Published on: June 6, 2011

25.0K
Author Spotlight: Developing Innovative Therapeutic Strategies for Hemorrhagic Shock Research
08:14

Author Spotlight: Developing Innovative Therapeutic Strategies for Hemorrhagic Shock Research

Published on: March 22, 2024

1.6K
Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring
06:11

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

Published on: May 21, 2017

8.7K

Area of Science:

  • Immunology
  • Trauma Research
  • Molecular Medicine

Background:

  • Trauma/hemorrhagic shock followed by resuscitation (T/HS-R) induces multi-system inflammation and organ dysfunction.
  • Toll-like Receptor 4 (TLR4) plays a role in mediating inflammatory responses to T/HS-R.
  • Type 17 immune cells are implicated in human trauma responses.

Purpose of the Study:

  • To investigate the role of TLR4 in the dynamic inflammatory response following experimental T/HS-R.
  • To define the protein-level inflammatory mediator dynamics across multiple tissues and circulation.
  • To assess the involvement of type 17 immune cells in T/HS-R and their relationship with TLR4.

Main Methods:

  • Experimental T/HS-R model in wild-type (WT) and TLR4-null (TLR4-/-) mice.
  • Quantification of 20 protein-level inflammatory mediators in 7 tissues and systemic circulation.
  • Statistical analyses including cross-correlation, Principal Component Analysis (PCA), and hypergraph analysis.

Main Results:

  • TLR4-/- mice showed distinct inflammatory mediator distribution compared to WT mice, with mediators localized to fewer compartments.
  • Correlations between IL-17A and other cytokines (GM-CSF, IL-10, TNF) indicated presence of different T helper 17 cell subsets in kidney and gut of both WT and TLR4-/- mice.
  • TLR4 appears to regulate the efflux of T helper 17 cells from circulation into tissues following T/HS-R, as evidenced by hypergraph and PCA analyses.

Conclusions:

  • TLR4 influences the spatial and temporal distribution of inflammatory mediators after T/HS-R.
  • TLR4 plays a role in controlling the trafficking of type 17 immune cells following trauma and resuscitation.
  • These findings suggest a complex, context-specific role for TLR4 and type 17 immunity in T/HS-R.