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Related Experiment Video

Updated: Sep 20, 2025

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics.

Muhammedin Deliorman1, Ayoub Glia1, Mohammad A Qasaimeh1,2

  • 1Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, UAE.

STAR Protocols
|June 6, 2022
PubMed
Summary
This summary is machine-generated.

This study presents the AFM-Chip, a microfluidic device for capturing rare circulating tumor cells (CTCs) and seamlessly integrating them with atomic force microscopy (AFM) for biomechanical analysis.

Keywords:
Atomic Force Microscopy (AFM)Biotechnology and bioengineeringCell isolationSingle Cell

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Microfluidics

Background:

  • Elasticity and bio-adhesiveness of circulating tumor cells (CTCs) are crucial cancer biomarkers.
  • CTCs are rare in blood, necessitating specialized capture and characterization techniques.

Purpose of the Study:

  • To describe the fabrication and utilization of a multifunctional microfluidic device, the AFM-Chip.
  • To enable efficient capture and seamless atomic force microscopy (AFM) based biomechanical characterization of CTCs.

Main Methods:

  • Fabrication of the AFM-Chip, a multifunctional microfluidic device.
  • Affinity-based capture of CTCs within the microfluidic device.
  • Reversible physical assembly for integrating the AFM-Chip with AFM for characterization.

Main Results:

  • The AFM-Chip facilitates efficient capture of CTCs.
  • Seamless transition from CTC capture to AFM characterization with minimal cell loss.
  • Demonstrated utility for biomechanical characterization of CTCs.

Conclusions:

  • The AFM-Chip is an effective tool for isolating and analyzing CTCs.
  • This integrated approach enhances the study of CTC biomechanics as cancer biomarkers.
  • The protocol provides a streamlined method for CTC analysis.