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Updated: Sep 20, 2025
Murine Fecal Isolation and Microbiota Transplantation
Published on: May 26, 2023
Fecal Microbiota and Human Intestinal Fluid Transplantation: Methodologies and Outlook
Ye Chen1, Lin Zhiliang1, Cui Jiaqu1
1Department of Colorectal Disease Specialty, Clinical Research Center for Digestive Diseases, The Tenth People's Hospital, Tongji University, Shanghai, China.
Abstract:
Fecal microbiota transplantation (FMT) is a therapy that involves the transplantation of healthy human fecal microorganisms into the gut of patients to rebuild or consolidate the intestinal microecology. It has been utilized in many diseases. However, FMT had a limited effect on patients with small intestinal diseases because of the unique ecological characteristics of the microorganisms. Thus, we proposed a new microecology transplantation therapy called human intestinal fluid transplantation (HIFT). Human intestinal fluid can be collected through a nasojejunal tube and be made into capsules using the freeze-dried powder method. In addition, strict standards for donor screening and management have been established. We are currently developing a high-standard HIFT preparation system and conducting high-quality clinical studies to validate the safety and efficacy of HIFT combined with FMT.
Insights
Human intestinal fluid transplantation (HIFT) is a novel therapy for small intestinal diseases, addressing limitations of fecal microbiota transplantation (FMT). HIFT utilizes processed intestinal fluid to restore gut microecology, with ongoing clinical studies validating its safety and efficacy.
Area of Science:
- Microbiology
- Gastroenterology
- Transplantation Medicine
Background:
- Fecal microbiota transplantation (FMT) is an established therapy for modulating gut microecology.
- FMT efficacy is limited in small intestinal diseases due to unique microbial characteristics.
- A novel approach is needed to address the microecological challenges in the small intestine.
Purpose of the Study:
- To introduce human intestinal fluid transplantation (HIFT) as a new therapeutic strategy.
- To describe the methodology for HIFT preparation and administration.
- To outline the development of a HIFT preparation system and clinical validation studies.
Main Methods:
- Human intestinal fluid collected via nasojejunal tube.
- Preparation of intestinal fluid into capsules using freeze-dried powder.
- Establishment of strict donor screening and management standards.
- Development of a high-standard HIFT preparation system.
- Conducting clinical studies to assess HIFT safety and efficacy.
Main Results:
- Human intestinal fluid transplantation (HIFT) is proposed as a novel therapy.
- HIFT preparation involves collection, freeze-drying, and encapsulation of intestinal fluid.
- Rigorous donor screening and management protocols are implemented.
- Clinical studies are underway to evaluate HIFT's safety and efficacy.
- HIFT aims to complement FMT for comprehensive microecological restoration.
Conclusions:
- Human intestinal fluid transplantation (HIFT) offers a promising alternative for small intestinal diseases.
- HIFT addresses the limitations of FMT in unique small intestinal environments.
- Ongoing research focuses on optimizing HIFT preparation and clinical application.
- HIFT, potentially combined with FMT, seeks to improve treatment outcomes for intestinal disorders.
