Development of Synthetic Riboswitches to Guide the Evolution of Metabolite Production in Microorganisms

The untranslated region (UTR) of prokaryotic mRNA contains riboswitches, which are gene regulating modules. Riboswitches can be used as biosensors to regulate the expression of a gene or an operon depending on the intracellular level of a target molecule and consequently modulate the cellular responses. In evolutionary engineering, riboswitch-based biosensors have been widely applied for high-throughput screening or selection of target phenotypes. Evolutionary approaches can overcome the limitations of rational approaches in metabolic engineering. Previous studies have reported synthetic riboswitches equipped with novel aptamers and marker genes based on a deep understanding of the operation mechanism of the riboswitch. Here, we introduce the development process of novel synthetic riboswitches for applications in metabolic engineering.