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Updated: Sep 20, 2025

Generation of Mice Derived from Induced Pluripotent Stem Cells
Published on: November 29, 2012
Generation of a human iPSC line (MPIi008-A) from a patient with Denys-Drash syndrome
Markus C Doeser1, Julia Krygin2, Albrecht Röpke3
1Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany; Interdisciplinary Center for Clinical Research (IZKF), University of Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany.
Abstract:
An induced pluripotent stem cell (hiPSC) line (MPIi008-A) was generated from fibroblasts of a 1-year-old male patient with Denys-Drash syndrome using lentiviral delivery of reprogramming factors OCT4, SOX2, KLF4 and c-MYC. The MPIi008-A iPSC line exhibited typical iPSC morphology and normal karyotype, expressed pluripotent stem cell markers, and showed developmental potential to differentiate into derivatives of all three germ layers in vivo. The hiPSC line harbours a heterozygous missense mutation (R394L) in exon 9 of the WT1 gene.
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