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Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment
Biochemical and Biophysical Research Communications
|March 13, 1987
Summary
Researchers identified a human liver-type pyruvate kinase (PK) cDNA clone. DNA sequencing and gene mapping confirmed the human L-type PK gene is located on chromosome 1.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Pyruvate kinase (PK) is a key enzyme in glycolysis, existing in four main isozymes (L, R, M1, M2).
- These isozymes are primarily encoded by two distinct genes, highlighting the complexity of PK gene regulation.
Purpose of the Study:
- To isolate and characterize a human cDNA clone encoding a liver-type pyruvate kinase (PK).
- To determine the chromosomal location of the human L-type PK gene.
Main Methods:
- Isolation of a human liver cDNA clone (hlPK-1) using a rat liver PK cDNA probe.
- DNA sequence analysis of the isolated cDNA insert.
- Karyotype analysis of human-mouse hybrid cells and Southern blot analysis to map the gene's location.
Main Results:
- The hlPK-1 clone contained a 1,049-base pair cDNA insert encoding the carboxyl-terminal 105 amino acids of human L-type PK and a 3' untranslated region.
- Sequence comparison with rat PK cDNAs confirmed its identity as human L-type PK.
- Gene mapping studies localized the human L-type PK gene to chromosome 1.
Conclusions:
- The study successfully isolated and sequenced a human L-type PK cDNA.
- The human L-type PK gene has been definitively mapped to chromosome 1, providing crucial information for understanding PK gene organization and potential genetic disorders.