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Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
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Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
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Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into complex structures.
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Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Related Experiment Video

Updated: Sep 20, 2025

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves
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Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

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CLASP balances two competing cell division plane cues during leaf development.

Liyong Zhang1, Chris Ambrose2

  • 1Department of Biology, The University of Saskatchewan, College of Arts and Science, Saskatoon, Saskatchewan, Canada.

Nature Plants
|June 6, 2022
PubMed
Summary
This summary is machine-generated.

Leaf mesophyll cell division patterns, regulated by microtubule orientation and CLASP, control tissue organization. Two competing mechanisms balance cell and air space distribution in Arabidopsis spongy mesophyll.

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Area of Science:

  • Plant biology
  • Cell biology
  • Developmental biology

Background:

  • Leaf mesophyll cells form an intricate mesh crucial for photosynthesis.
  • Understanding mesophyll cell division patterns is key to their organization and intercellular space formation.

Purpose of the Study:

  • To investigate the basic patterns of mesophyll cell division.
  • To understand how these patterns contribute to cell and intercellular space organization in Arabidopsis spongy mesophyll.

Main Methods:

  • Tracking of individual cell lineages in 3D over time.
  • Analysis of microtubule orientation and preprophase band formation.
  • Investigating the role of CLASP using genetic mutants.

Main Results:

  • Early cell divisions feature alternating perpendicular planes, forming multi-cell junctions that open into intercellular spaces.
  • Later divisions tilt towards existing spaces, promoting expansion rather than new space formation.
  • CLASP is essential for balancing these division patterns, preventing distortions in tissue topology.

Conclusions:

  • Two competing cell division mechanisms govern mesophyll tissue organization.
  • CLASP acts as a crucial regulator, balancing these mechanisms to determine cell and intercellular space distribution.
  • Microtubule orientation dictates division plane specification prior to mitosis.