Composite Superelastic Aerogel Scaffolds Containing Flexible SiO₂ Nanofibers Promote Bone Regeneration

Repairing irregular-shaped bone defects poses enormous challenges. Scaffolds that can fully fit the defect site and simultaneously induce osteogenesis and angiogenesis hold great promise for bone defect healing. This study aimed to produce superelastic organic/inorganic composite aerogel scaffolds by blending silica nanofibers (SiO₂ ) and poly (lactic acid)/gelatin (PLA/gel) nanofibers; the content of SiO₂ nanofibers is varied from 0-60 wt% (e.g., PLA/gel, PLA/gel/SiO₂ -L, PLA/gel/SiO₂ -M, and PLA/gel/SiO₂ -H for 0%, 20%, 40%, and 60% of SiO₂ nanofibers, respectively) to produce a range of scaffolds. The PLA/gel/SiO₂ -M scaffold has excellent elasticity and good mechanical properties. In vitro experiments demonstrate that the silicon ions released from PLA/gel/SiO₂ -M scaffolds promote the differentiation of rat bone marrow-derived mesenchymal stem cells into osteoblasts, enhancing alkaline phosphatase activity and bone-related genes expressions. The released silicon ions also promote the proliferation of human umbilical vein endothelial cells and the expression of vascular endothelial growth factors, thereby promoting angiogenesis. The assessment of these scaffolds in a calvarial defect model in rats shows good potential of PLA/gel/SiO₂ -M to induce bone regeneration as well as promote osteogenesis and angiogenesis. Overall, these organic/inorganic composite scaffolds have good biological activity, which may have broad applications for tissue engineering.