Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ambient Light-Activatable Luminescent Particle-Embedded Conformal Patch for Photochemical Tissue Bonding and Photobiomodulated Healing.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Structure, Interactions, and Assembly of Membrane-Active Antimicrobial Polypeptides.

Chemical reviews·2026
Same author

Probing Interactions of Plastic Nanoparticles with Biomolecular Systems via Molecular Simulations.

Nano letters·2026
Same author

Bionano Interface Optimization for Rational Lateral Flow Assay Development.

ACS nano·2026
Same author

Microfluidic Encapsulation of Genetically Engineered Bone-marrow-derived Mesenchymal Stem Cells for Bone Defect Healing.

Advanced healthcare materials·2026
Same author

Engineering Temperature-Switchable Conducting Metal-Phenolic Network Films.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Jun 9, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
10:53

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions

Published on: October 10, 2016

14.2K

Block Length-Dependent Protein Fouling on Poly(2-oxazoline)-Based Polymersomes: Influence on Macrophage Association

Adrian Najer1, Alexis Belessiotis-Richards1, Hyemin Kim1

  • 1Department of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.

Small (Weinheim an Der Bergstrasse, Germany)
|June 7, 2022
PubMed
Summary

This study optimized polymersomes for medical use by investigating poly(2-oxazoline) (POx) properties. Maximizing POx length and using copolymer blends improved stability and reduced protein fouling, enhancing their potential for drug delivery.

Keywords:
atomistic simulationsnanoparticlesprotein coronaprotein foulingzebrafish embryos

More Related Videos

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.5K
Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
06:01

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

Published on: April 21, 2021

3.3K

Related Experiment Videos

Last Updated: Jun 9, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
10:53

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions

Published on: October 10, 2016

14.2K
A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.5K
Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
06:01

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

Published on: April 21, 2021

3.3K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Polymersomes offer promise as alternatives to liposomes in various biomedical applications.
  • Clinical translation of polymersomes is hindered by limited data on stability, protein fouling, and blood circulation.
  • Poly(2-oxazoline)s (POx) show potential as antifouling agents, but their nanoparticle applications require further investigation.

Purpose of the Study:

  • To systematically investigate the structural, dynamic, and antifouling properties of poly(2-methyl-2-oxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PMOXA-b-PDMS-b-PMOXA) polymersomes.
  • To correlate in vitro antifouling performance with molecular dynamics simulations of membrane hydration.
  • To establish design principles for stable, low-fouling polymersomes for medical applications.

Main Methods:

  • Synthesis of PMOXA-b-PDMS-b-PMOXA polymersomes with varying block lengths.
  • Atomistic molecular dynamics simulations to study membrane hydration.
  • In vitro assessment of protein fouling and macrophage association.
  • In vivo blood circulation studies in zebrafish embryos.

Main Results:

  • Optimal polymersome stability and antifouling properties were achieved by maximizing PMOXA length (DP > 6) and minimizing PDMS length (DP > 19).
  • Molecular dynamics simulations supported experimental findings on membrane hydration and antifouling.
  • Polymersomes with blends of varied copolymer lengths demonstrated superior performance over single copolymer presentations.
  • In vitro and in vivo studies confirmed reduced macrophage association and improved blood circulation for optimized polymersomes.

Conclusions:

  • The study provides rational design rules for developing stable and low-fouling polymersomes.
  • Optimized PMOXA-based polymersomes show significant potential for advanced medical applications, including drug delivery and immunotherapy.
  • Understanding the interplay between polymer structure and interfacial properties is crucial for next-generation nanomedicine.