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Yellow Fever

Yellow Fever

Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...

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Updated: Jun 16, 2026

Optimization of a Quantitative Micro-neutralization Assay

Optimization of a Quantitative Micro-neutralization Assay

Published on: December 14, 2016

A High-Throughput Yellow Fever Neutralization Assay.

Madina Rasulova^1,2, Thomas Vercruysse^1,2, Jasmine Paulissen^1,2

¹KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.

Microbiology Spectrum

|June 7, 2022

Summary

This summary is machine-generated.

A new fluorescence-based assay (SNT^FLUO) offers fast, accurate detection of yellow fever virus neutralizing antibodies (nAbs). This high-throughput method matches the gold standard

Keywords:

Japanese encephalitis virus PRNT Zika virus dengue virus high-throughput neutralization assay reporter virus serodiagnosis yellow fever virus

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Last Updated: Jun 16, 2026

Optimization of a Quantitative Micro-neutralization Assay

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Published on: December 14, 2016

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

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Published on: April 10, 2018

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

Area of Science:

Virology
Immunology
Public Health

Background:

Accurate detection of yellow fever virus neutralizing antibodies (nAbs) is crucial for serodiagnosis, outbreak surveillance, and vaccine efficacy assessment.
The traditional plaque reduction neutralization test (PRNT) is sensitive and specific but laborious, time-consuming, and has low throughput.
There is a need for high-throughput, standardized serological assays for yellow fever virus (YFV) antibody detection.

Purpose of the Study:

To develop and validate a novel fluorescence-based serum neutralization test (SNT^FLUO) for high-throughput detection of YFV nAbs.
To ensure the developed assay demonstrates sensitivity and specificity comparable to the gold standard PRNT.
To assess the potential for clinical implementation and automation of the SNT^FLUO assay.

Main Methods:

Development of a fluorescence-based serum neutralization test (SNT^FLUO).
Validation of SNT^FLUO against the gold standard plaque reduction neutralization test (PRNT).
Cross-validation of the assay in multiple reference laboratories and against international WHO standards.

Main Results:

The SNT^FLUO assay demonstrated high sensitivity and specificity, comparable to the PRNT.
The assay is suitable for high-throughput testing and has potential for automation.
Validated SNT^FLUO assays are available for Japanese encephalitis, Zika, and dengue viruses, enabling differential diagnostics.

Conclusions:

The novel SNT^FLUO assay provides a sensitive, specific, and high-throughput alternative for detecting yellow fever virus neutralizing antibodies.
The assay's performance and potential for automation make it suitable for clinical use and large-scale studies.
Availability of similar SNT^FLUO assays for related flaviviruses facilitates differential diagnosis.