Related Experiment Video

Updated: Sep 20, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

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CCAR2 controls mitotic progression through spatiotemporal regulation of Aurora B

Jaewook Ryu1, Ja-Eun Kim2,3

  • 1Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, Korea.

Cell Death & Disease
|June 7, 2022
PubMed

Insights

Cell cycle and apoptosis regulator 2 (CCAR2) deficiency impairs cell division, causing errors in chromosome segregation and halting cytokinesis. This study reveals CCAR2

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell cycle and apoptosis regulator 2 (CCAR2) is known for its role in cell survival and death.
  • Its physiological functions in regulating normal cell proliferation are not well understood.

Purpose of the Study:

  • To investigate the role of CCAR2 in cell proliferation and mitosis in the absence of external stimuli.
  • To elucidate the molecular mechanisms underlying CCAR2's function in cell division.

Main Methods:

  • Generation and analysis of CCAR2-deficient cells.
  • Microscopy to observe nuclear morphology and mitotic progression.
  • Analysis of centromere cohesion, spindle assembly checkpoint, and abscission checkpoint.
  • Investigation of Aurora B kinase activity regulation.

Main Results:

  • CCAR2-deficient cells exhibit multilobulated nuclei, indicating cell division defects.
  • Mitotic phase duration is perturbed due to premature loss of centromere cohesion and spindle assembly checkpoint inactivation.
  • Lagging chromosomes and abscission checkpoint activation were observed, halting cytokinesis.
  • CCAR2 regulates mitotic progression through spatiotemporal control of active Aurora B.

Conclusions:

  • CCAR2 is essential for normal mitotic progression, including chromosome segregation and cytokinesis.
  • CCAR2 plays a critical role in maintaining chromosomal stability.
  • The findings highlight CCAR2's novel function in regulating cell division independently of cytotoxic stress.

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