Related Experiment Video
Updated: Sep 20, 2025
A Simple Composite Phenotype Scoring System for Evaluating Mouse Models of Cerebellar Ataxia
Published on: May 21, 2010
Hepatitis, testicular degeneration, and ataxia in DIDO3-deficient mice with altered mRNA processing
Julio Gutiérrez1, Karel H M van Wely2, Carlos Martínez-A2
1Department of Immunology and Oncology, Centro Nacional de Biotecnología-CSIC, Darwin 3, 28049, Madrid, Spain. jgutierrez@cnb.csic.es.
Background:
mRNA processing is an essential step of gene expression; its malfunction can lead to different degrees of physiological disorder from subclinical disease to death. We previously identified Dido1 as a stemness marker and a gene involved in embryonic stem cell differentiation. DIDO3, the largest protein encoded by the Dido1 gene, is necessary for accurate mRNA splicing and correct transcription termination. The deletion of Dido1 exon16, which encodes the carboxy-terminal half of DIDO3, results in early embryonic lethality in mouse.
Results:
We obtained mice bearing a Cre-LoxP conditional version of that deletion and studied the effects of inducing it ubiquitously in adult stages. DIDO3-deficient mice survive the deletion but suffer mild hepatitis, testicular degeneration, and progressive ataxia, in association with systemic alterations in mRNA splicing and transcriptional readthrough.
Conclusions:
These results offer insight into the distinct vulnerabilities in mouse organs following impairment of the mRNA processing machinery, and could aid understanding of human health dependence on accurate mRNA metabolism.
